| HSD17b3

17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) is an enzyme that is encoded by the HSD17B3 gene and is involved in androgen steroidogenesis.This isoform of 17β-HSD is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in impaired virilization of genetically male infants, formerly termed male pseudohermaphroditism.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.48	1.65	1.07	0.71	0.45	0.28	0.19	0.09
Corrected OD	2.39	1.56	0.98	0.62	0.36	0.19	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat HSD17b3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	83-101	96
Cell Lysates(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Rat HSD17b3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	86-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	96	98